Jonathan R. Brestoff, MD, PhD, MPH

Jonathan R. Brestoff, MD, PhD, MPH

Assistant Professor, Pathology & Immunology


  • Laboratory & Genomic Medicine


  • BS: Skidmore College (2008)
  • MPH: University College Cork – National University of Ireland, Cork (2010)
  • MD/PhD: Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, (2016)
  • Residency: Clinical Pathology, Barnes-Jewish Hospital/SLCH/Washington University School of Medicine (2019)

Related Links

Board Certification

  • American Board of Pathology, Clinical Pathology


  • Barry M. Goldwater Scholarship
  • George J. Mitchell Scholarship
  • Joseph C. Palamountain Award for Young Alumni Achievement, Skidmore College
  • Roy G. Williams Award, University of Pennsylvania
  • Endocrine Society Award for Medical Student Achievement
  • Paul E. Strandjord Young Investigator Award, ACLPS
  • William L. Roberts Award with Distinction, ACLPS
  • Children’s Discovery Institute Fellowship
  • Career Award for Medical Scientists, Burroughs Welcome Fund
  • NIH Director’s Early Independence Award (DP5)

Clinical Interests

  • Immunology diagnostic testing
  • Implementation of spectral flow cytometry for clinical use

Research Interests

The immune system is able to control the function of various metabolic organs and contributes to metabolic abnormalities seen in diseases such as obesity and type 2 diabetes. The Brestoff Lab’s research focuses on understanding how immune cells and metabolic organs interact to maintain normal metabolism and to support adaptation to physiologic challenges such as high-fat diet feeding, changes in environmental temperature, and fasting. We employ cutting-edge techniques in immunology, metabolism, and mitochondria biology to study the immunometabolic pathways that are required for metabolic health and that contribute to disease pathogenesis. Ultimately, our goal is to discover novel biological pathways that can be targeted therapeutically or that can be leveraged to develop new diagnostic tests.

Selected Publications

Brestoff JR, Wilen CB, Moley JR, Li Y, Zou W, Malvin NP, Rowen MN, Saunders BT, Ma H, Mack MR, Hykes BL, Balce DR, Orvedahl A, Williams JW, Rohatgi N, Wang X, McAllaster MR, Handley SA, Kim BS, Doench JG, Zinselmeyer BH, Diamond MS, Virgin HW, Gelman AE, Teitelbaum SL. Intercellular mitochondria transfer to macrophages regulates white adipose tissue homeostasis and is impaired in obesity. Cell Metaboslim2021. Epub ahead of print.
Wilen CB, Lee S, Hsieh LY, Orchard RC, Desai C, Hykes BL Jr, McAllaster MR, Balce DR, Feehley T, Brestoff JR, Hickey CA, Yokoyama CC, Zhang X, Wang YT, MacDuff DA, Kreamalmayer D, Howitt MR, Cadwell K, Allen PM, Handley SA, Campagne MvL, Baldridge MT, Virgin HW. Tropism for tuft cells determines immune promotion of norovirus pathogenesis. Science. 2018; 360(6385): 204-208.
Moriyama S, Brestoff JR, Flamar AL, Moeller JB, Klose CSN, Rankin LC, Yudanin NA, Monticelli LA, Putzel GG, Rodewald HR, Artis D. 2-adrenergic receptor-mediated negative regulation of group 2 innate lymphoid cell responses. Science. 2018; 359(6379): 1056-1061.
Brestoff JR, Tesfazghi M, Zaydman MA, Jackups Jr R, Kim BS, Scott MG, Gronowski AM, Grossman BJ. The B antigen protects against the development of red meat allergy. J Allergy Clin Immunol Pract. 2018; 6(5): 1790-1791.e3. #Corresponding author.
Oetjen LK, Mack MR, Whelan TM, Wang PL, Niu H, Feng J, Xu AZ, Tripathi SV, Guo CJ, Luo J, Brestoff JR, Schaffer A, Hsieh C, Gereau RW, Miller MJ, Hu H, Davidson S, Liu Q and Kim BS. Neuronal IL-4Rα and JAK1 signaling mediate chronic itch. Cell. 2017; 171(1): 217-228.
Williams J*, Elvington AF*, Ivanov S*, Kessler S, Luehmann H, Baba O, Saunders BT, Kim K, Johnson MW, Craft CS, Choi J, Sorci-Thomas MG, Zinselmeyer BH, Brestoff JR, Liu Yongjian, Randolph GJ. Thermoneutrality but not UCP1 deficiency suppresses monocyte mobilization into blood. Circ Res. 2017; 121(6): 662-676. *Equal contributions
Brestoff JR, Zaydman MA, Scott MG and Gronowski AM. Diagnosis of red meat allergy with antigen-specific IgE tests in serum. J Allergy Clin Immunol. 2017; 140(2): 608-609. #Corresponding author.
Brestoff JR and Artis D. Immune cell regulation of metabolic homeostasis in health and disease. Cell. 2015; 161(1): 146-160.
Brestoff JR, Kim BS, Saenz SA, Stine RR, Monticelli LA, Sonnenberg GF, Thome JJ, Farber DL, Lutfy K, Seale P, Artis D. Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity. Nature. 2015; 519: 242-246.
Brestoff JR and Artis D. Commensal bacteria at the interface of host metabolism and the immune system. Nat Immunol. 2013; 14(7): 676-684.

Dionne Brierton
BJCIH Room 5800