Overview
The Pathology Transgenic, Knockout and Mouse Core offers a wide array of mouse embryo manipulation techniques. We provide investigators a resource to generate mutant mouse models through a variety of techniques, most recently focusing on CRISPR mouse models. We average 80-100 CRISPR models per year and have completed over 900 CRISPR projects. In addition to producing genetically edited mouse models, we also offer embryo or sperm cryo-preservation and storage, embryo rederivation, frozen sample recovery, and IVF. C57BL/6J inbred is our standard strain for generation of mouse models. We also offer Albino B6 host embryos for injection of B6 ES cell lines. We have proven success using several other strains including F1 hybrid strains, NOD, FVB, Balb/c, CBA, DBA, and C3He. We can also provide consultation model development, assist with MEF production and ES culture in your lab and are a resource for many mouse related topics. We can apply our techniques in a variety of ways to help benefit your research. Letters of support are also available for grant writing needs.
Phone: 314-362-1182 | 314-747-3267 (Injection Lab)
Email: white@wustl.edu
Contacts
Services
Service available to: All entities, including for-profit organizations.
Priority service given to: WashU Medicine
C57Bl/6 is standard strain, others possible; contact Core Director for more information.
For knock out/knock in production. Targeted ES cells provided by individual labs or through the cancer center, etc.
ES cells injected into RAG 2 knock out blastocysts to complement the RAG 2 phenotype for study of T and B cells within a live animal derived from your mutant ES cells.
ES cells aggregated with or injected into tetraploid embryos. Produces embryos or live mice that are completely ES cell derived.
Cleans up lines infected with various parasites or pathogens.
Assisted in your laboratory (available, time permitting)
Assisted in your laboratory (available, time permitting)
Immunobiology
The Division of Immunobiology is one of five units within the Department of Pathology & Immunology, and is committed to cutting-edge, leading research in basic immunology.
The division was established in 2006 in recognition of the long and distinguished history of immunology research in our department. With 14 basic science laboratories, including four members of the National Academy of Sciences, the division is internationally recognized for its key discoveries in immunology.