Overview
The Transgenic, Knockout and Micro-Injection Core offers a wide array of mouse embryo manipulation techniques. We provide investigators a resource to generate mutant mouse models through a variety of techniques, cryopreserve mouse lines, cryopreservation/storage of mouse sperm, perform embryo rederivation, thaw embryos and perform IVF. C57BL/6 inbred is our standard strain for both pronuclear and ES micro-injection, we also offer Albino B6 host embryos for injection of B6 ES cell lines such as those from KOMP, EUCOMM TIGM and other cell suppliers. Hybrids and additional inbred strains may be used for transgenic production. We thaw/reconstitute embryos from many different sources and perform IVF.
Phone: 314-362-1182 | 314-747-3267 (Injection Lab)
Email: white@wustl.edu
Contacts
Services
Service available to: All entities, including for-profit organizations.
Priority service given to: WashU Medicine
C57Bl/6 is standard strain, others possible; contact Core Director for more information.
For knock out/knock in production. Targeted ES cells provided by individual labs or through the cancer center, etc.
ES cells injected into RAG 2 knock out blastocysts to complement the RAG 2 phenotype for study of T and B cells within a live animal derived from your mutant ES cells.
ES cells aggregated with or injected into tetraploid embryos. Produces embryos or live mice that are completely ES cell derived.
Cleans up lines infected with various parasites or pathogens.
Assisted in your laboratory (available, time permitting)
Assisted in your laboratory (available, time permitting)
Immunobiology
The Division of Immunobiology is one of five units within the Department of Pathology & Immunology, and is committed to cutting-edge, leading research in basic immunology.
The division was established in 2006 in recognition of the long and distinguished history of immunology research in our department. With 14 basic science laboratories, including four members of the National Academy of Sciences, the division is internationally recognized for its key discoveries in immunology.