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Research > Faculty By Division > Erika Crouch, MD, PhDProfessor, Pathology and Immunology
Faculty, Division of Biology and Biomedical Sciences
Course Leader, Sophomore Pathology Course
Room 5816, BJC-IH, 5th Floor Suite 5801
Office: (314) 454-8462
Lab: (314) 454-7577
E-mail: crouch@wustl.edu
Web-site: http://pathology.wustl.edu/~crouchlab
Pub Med Search |  |
Research
| | Our research has focused on the structure and function of collagenous carbohydrate binding proteins known as collectins. We have systematically characterized the structure, function, synthesis, assembly and secretion of Surfactant Protein D (SP-D) - a collectin that plays important roles in the innate pulmonary host defense against a wide variety of important bacterial, fungal, and viral pathogens. In particular, the laboratory has employed site-directed mutagenesis and crystallographic analysis to examine mechanisms of microbial pattern recognition, including interactions with the hemagglutinin of influenza A. |
Service to the Department
| | 2010 - Present | CLIA Laboratory Director, AMP Core Labs | | 2009 - Present | Director, Center for Clinical and Scientific Studies | | 2007 - Present | Clinician, Siteman Cancer Center, Barnes-Jewish Hospital and Washington University School of Medicine | | 1996 - Present | Course Director, Sophomore Pathology Course |
Service to the University
| | 2009 - 2010 | President of WUSM Chapter of AOA | | 2008 - 2011 | Educational Technology Advisory Committee | | 2008 - 2009 | Vice President, WUSM Chapter of AOA | | 2006 - 2007 | Educational Programs Committee, LCME Self Study | | 2006 - 2008 | Information Technology Focus Group, WUSM | | 2005 - 2011 | Selection Committee, WUSM Chapter of AOA | | 2005 - Present | Loeb Teaching Fellowship Selection Committee (Appointed by Dean) | | 2004 - Present | Chair, Goldstein Education Committee (Appointed by Dean) | | 2004 - 2005 | Preclinical Infrastructure, Devices, and Related Software Subcommittee, WUSM | | 2004 - 2005 | Taskforce for Information Technology in Medical Student Education, WUSM | | 2001 - 2003 | Learning and Teaching Center Committee, WUSM | | 2000 - 2006 | Information Technology in Medical Student Education Committee, WUSM | | 1999 - Present | Coordinator and Lecturer, Pulmonary Fellows Pathology Conference | | 1999 - 2000 | Executive Faculty Committee on Information Technology, WUSM | | 1999 - 2000 | Chair, Education Committee for Faculty Retreat | | 1998 - 2004 | Medical Student Advisory Program | | 1998 - 2005 | Committee on Medical Education (CME), WUSM | | 1997 - Present | Committee on Academic and Professional Evaluation of Students (CAPES), WUSM | | 1997 - Present | Curriculum Evaluation Committee II (CEC II), WUSM | | 1997 - 1998 | LCME Objectives Committee, WUSM | | 1986 - Present | Faculty and Internal Advisory Committee, Pulmonary Research Training Grant |
Diagnostic Expertise
| | Breast Pathology | | Pulmonary Pathology - particularly interstitial and occupational lung diseases |
Clinical Interest
| | Anatomic and Molecular Pathology - Breast Pathology | | Anatomic and Molecular Pathology - Immunohistochemistry | | Anatomic and Molecular Pathology - Pulmonary Pathology |
Selected Publications
| | Selected from 207 Peer Reviewed Publications. . , 2013
| | Tripathi S, Tecle T, Verma A, Crouch E, White M, Hartshorn KL. The human cathelicidin LL-37 inhibits influenza A viruses through a mechanism distinct from that of Surfactant Protein D or definsins. J Gen Virol 94(Pt 1):40-49, 2013 Abstract
| | van Eijk M, Rynkiewicz MJ, White MR, Hartshorn KL, Zou X, Schulten K, Luo D, Crouch EC, Cafarella TR, Head JF, Haagsman HP, Seaton BA. A Unique Sugar-binding Site Mediates the Distinct Anti-influenza Activity of Pig Surfactant Protein D. J Biol Chem 287:26666-26677, 2012 Abstract
| | Stolley JM, Gong D, Farley K, Zhao P, Cooley J, Crouch EC, Benarafa C, Remold-O'Donnell E. Increased Surfactant Protein D fails to improve bacterial clearance and inflammation in serpinB1-/- mice.. Am J Respir Cell Mol Biol 47(6):792-799, 2012 Abstract
| | Doss M, Ruchala P, Tecle T, Gantz D, Verma A, Hartshorn A, Crouch EC, Luong H, Micewicz ED, Lehrer RI, Hartshorn KL. Hapivirins and Diprovirins: Novel theta-defensin analogs with potent activity against Influenza A Virus.. J Immunol 188(6):2759-2768, 2012 Abstract
| | Qi L, Kash JC, Dugan VG, Jagger BW, Lau YF, Sheng ZM, Crouch EC, Hartshorn KL, Taubenberger JK. The ability of pandemic influenza virus hemagglutinins to induce lower respiratory pathology is associated with decreased Surfactant Protein D binding. Virology 412(2):426-434, 2011 Abstract
| | Crouch E, Nikolaidis N, McCormack F, McDonald B, Allen K, Rynkiewicz M, Cafarella T, White M, Lewnard K, Leymarie N, Zaia J, Seaton B, Hartshorn K. Mutagenesis of SP-D informed by evolution and xray crystallography enhances defenses against influenza A Virus in vivo. J Biol Chem 286:40681-40692, 2011 Abstract
| | Hartshorn KL, White MR, Tecle T, Sorensen G, Holmskov U, Crouch EC. Viral aggregating and opsonizing activity in collectin trimers. Am J Physiol Lung Cell Mol Physiol 298(1):L79-L88, 2010 Abstract
| | Lin KW, Jen KY, Suarez CJ, Crouch EC, Perkins DL, Finn PW. Surfactant Protein D-mediated decrease of allergen-induced inflammation is dependent upon CTLA4. J Immunol 184(11):6343-6349, 2010 Abstract
| | Hartshorn KL, White MR, Kuroki Y, Sorensen G, Holmskov U, Crouch EC. Increasing antiviral activity of Surfactant Protein D trimers by introducing residues from bovine serum collectins. Scan J Immunol 72(1):22-30, 2010 Abstract
| | Seaton BA, Crouch EC, McCormack FX, Head JF, Hartshorn KL, Mendelsohn R. Structural determinants of pattern recognition by lung collectins. Innate Immun 16(3):143-150, 2010 Abstract
| | Hartshorn KL, White MR, Rynkiewicz M, Sorensen G, Holmskov U, Head J, Crouch EC. Monoclonal antibody-assisted structure-function analysis of the carbohydrate recognition domain of Surfactant Protein D. Am J Physiol Lung Cell Mol Physiol 299(3):L384-L392, 2010 Abstract
| | Job ER, Deng YM, Tate MD, Bottazzi B, Crouch EC, Dean MM, Mantovani A, Brooks AG, Reading PC. Pandemic H1N1 Influenza A viruses are resistant to the antiviral activities of innate immune proteins of the collectin and pentraxin superfamilies. J Immunol 185(7):4284-4291, 2010 Abstract
| | Crouch EC, Hirche TO, Boxio R, Wartelle J, Benabid R, McDonald B, Heinecke J, Matalon S, Belaaouaj A. Myeloperoxidase-dependent inactivation of Surfactant Protein D in vitro and in vivo. J Biol Chem 285(22):16757-16770, 2010 Abstract
| | Matalon S, Shestra K, Kirk M, Waldheuser S, McDonald B, Smith K, Gao Z, Belaaouaj A, Crouch EC. Modification of Surfactant Protein D by reactive oxygen nitrogen intermediates is accompanied by loss of aggregating activity, in vitro and in vivo. FASEB J 23(5):1415-1430, 2009 Abstract
| | Doss M, White MR, Tecle T, Gantz D, Crouch EC, Jung G, Ruchala P, Waring AJ, Lehrer RI, Hartshorn KL. Interactions of alpha-, beta-, and theta-definsins with Influenza A virus and Surfactant Protein D. J Immunol 182:7878-7887, 2009 Abstract
| | Guth AM, Janssen WJ, Bosio CM, Crouch EC, Henson PM, Dow SW. Lung environment determines unique phenotype of alveolar macrophages. Am J Physiol Lung Cell Mol Physiol 296:L936-L946, 2009 Abstract
| | Crouch E, Hartshorn K, Horlacher T, McDonald B, Smith K, Cafarella T, Seaton B, Seeberger PH, Head J. Recognition of mannosylated ligands and influenza A virus by human Surfactant Protein D: contributions of an extended site and residue 343. Biochemistry 48(15):3335-3345, 2009 Abstract
| | Singh M, Madan T, Waters P, Sonar S, Singh SK, Kamran MF, Bernal AL, Sarma PU, Singh VK, Crouch EC, Kishore U. Therapeutic effects of recombinant forms of full-length and truncated human Surfactant Protein D in a murine model of invasive pulmonary aspergillosis. Mol Immunol 46(11-12):2363-2369, 2009 Abstract
| | Sorensen GL, Hoegh SV, Leth-Larsen R, Thomsen TH, Floridon C, Smith K, Kejling K, Tornoe I, Crouch EC, Holmskov U. Multimeric and trimeric subunit SP-D are interconvertible structures with distinct ligand interaction. Mol Immunol 46(15):3060-3069, 2009 Abstract
| | Carlson TK, Torrelles JB, Smith K, Horlacher T, Castelli R, Seeberger PH, Crouch EC, Schlesinger LS. Critical role of amino acid position 343 of Surfactant Protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis. Glycobiology 19(12):1473-1484, 2009 Abstract
| | White MR, Boland P, Tecle T, Gantz D, Sorenson G, Tornoe I, Holmskov U, McDonald B, Crouch EC, Hartshorn KL. Enhancement of antiviral activity of collectin trimers through cross-linking and mutagenesis of the carbohydrate recognition domain. J Innate Immun 2(3):267-279, 2009 Abstract
| | Wang H, Head J, Kosma P, Brade H, Muller-Loennies S, Sheikh S, McDonald B, Smith K, Cafarella T, Seaton B, Crouch E. Recognition of heptoses and the inner core of bacterial lipopolysaccharides by Surfactant Protein D. Biochemistry 47(2):710-720, 2008 Abstract
| | Nie X, Nishitani C, Yamazoe M, Ariki S, Takahashi M, Shimizu T, Mitsuzawa H, Sawada K, Smith K, Crouch E, Nagae H, Takahashi H, Kuroki Y. Pulmonary Surfactant Protein D binds MD-2 through the carbohydrate recognition domain. Biochemistry 47:12878-12885, 2008 Abstract
| | White M, Kingma P, Tecle T, Kacak N, Linders B, Heuser J, Crouch E, Hartshorn K. Multimerization of Surfactant Protein D, but not its collagen domain, Is required for antiviral and opsonic activities related to influenza virus. J Immunol 181(11):7936-7943, 2008 Abstract
| | Guo CJ, Atochina-Vasserman EN, Abramova E, Foley JP, Zaman A, Crouch E, Beers MF, Savani RC, Gow AJ. S-nitrosylation of Surfactant Protein D controls inflammatory function. PLoS Biol 6(11):2414-2423, 2008 Abstract
| | Hartshorn KL, Webby R, White MR, Tecle T, Pan C, Boucher S, Moreland RJ, Crouch EC, Scheule RK. Role of viral hemagglutinin glycosylation in anti-influenza activities of recombinant Surfactant Protein D. Respir Res 9:65, 2008 Abstract
| | Cooley J, McDonald B, Accurso F, Crouch E, Remold-O'Donnell E. Patterns of neutrophil serine protease-dependent cleavage of Surfactant Protein-D in inflammatory lung disease. J Leukoc Biol 83(4):946-955, 2008 Abstract
| | Sahly H, Kesari Y, Crouch E, Sharon N, Ofek I. Recognition of bacterial surface polysaccharides by lectins of the innate immune system and its contribution to defense against infection: the case of pulmonary pathogens. Infec Immun 76(4):1322-1332, 2008 Abstract
| | Reading PC, Bozza S, Gilbertson B, Tate M, Moretti S, Crouch EC, Brooks, AG, Brown LE, Bottazzi B, Ramani L, Mantovani A. Antiviral activity of the long chain pentraxin PTX3 against influenza viruses. J Immunol 180(5):3391-3398, 2008 Abstract
| | Tecle T, White MR, Sorensen GL, Gantz D, Kacak N, Holmskov U, Smith K, Crouch EC, Hartshorn KL. Critical role of crosslinking of trimeric lectin domains of Surfactant Protein D in antiviral activity against influenza A virus. Biochem J 412(2):323-329, 2008 Abstract
| | Wang L, Brauner JW, Mao G, Crouch E, Seaton B, Head J, Smith K, Flach CR, Mendelsohn R. Interaction of recombinant Surfactant Protein D with lipopolysaccharide: Conformation and orientation of bound protein by IRRAS and simulations. Biochem 47(31):8103-8113, 2008 Abstract
| | Hartshorn KL, White MR, Tecle T, Tornoe I, Sorensen GL, Crouch EC, Holmskov U. Reduced influenza viral neutralizing activity of natural human trimers of Surfactant Protein D. Respir Res 8:9 (5 Feb), 2007 Abstract
| | White MR, Tecle T, Crouch EC, Hartshorn KL. Impact of neutrophils on antiviral activity of human bronchoalveolar lavage fluid. Am J Physiol Lung Cell Mol Physiol 293(5):L1293-1299, 2007 Abstract
| | Crouch E, McDonald B, Smith K, Roberts M, Mealy T, Seaton B, Head J. Critical role of Arg/Lys343 in the species-dependent recognition of phosphatidylinositol by pulmonary Surfactant Protein D. Biochemistry 46:5160-5169, 2007 Abstract
| | Tecle T, White M, Crouch E, Hartshorn K. Human neutrophil defensins increase neutrophil uptake of influenza A virus and bacteria and modify virus induced respiratory burst responses. J Immunol 178(12):8046-8052, 2007 Abstract
| | Tecle T, White MR, Crouch EC, Hartshorn KL. Inhibition of influenza viral neuraminidase activity by collectins. Arch Virol 152(9):1731-1742, 2007 Abstract
| | Ferguson JS, Martin JL, Azad AK, McCarthy, TR, Kang PB, Voelker DR, Crouch EC, Schlesinger LS. Surfactant Protein D increases fusion of Mycobacterium tuberculosis-containing phagosomes with lysosomes in human macrophages.. Infect Immun 74(12):7005-7009, 2006 Abstract
| | Crouch EC, McDonald B, Smith K, Cafarella T, Seaton B, Head J. Contributions of phenylalanine 335 to ligand recognition by human Surfactant Protein D: Ring interactions with SP-D ligands. J Biol Chem 281(26):18008-18014, 2006 Abstract
| | Haczku A, Cao Y, Vass G, Kierstein S, Nath P, Atochina-Vasserman EN, Scanlon ST, Li L, Griswold DE,Chung KF, Pulain FR, Hawgood S, Beers MF, Crouch EC. IL-4 and IL-13 form a negative feedback circuit with SP-D in the allergic airway response. J Immunol 176(6):3557-3565, 2006 Abstract
| | Hartshorn KL, White MR, Tecle T, Holmskov U, Crouch EC. Innate defense against Influenza A virus: Activity of human neutrophil defensins and interactions of defensins with Surfactant Protein D. J Immunol 176(11):6962-6972, 2006 Abstract
| | Crouch EC. Surfactant Protein D (SP-D). In Encyclopedia of Respiratory Medicine. Chapter: Surfactant. G. Laurent and S. Shapiro, Ed, Elsevier Limited, Oxford, UK, Vol 4:152-158, 2006
| | Ohya M, Nishitani C, Sano H, Yamada C, Hiroki M, Shimizu T, Saito T, Smith K, Crouch E, Kuroki Y. Human pulmonary Surfactant Protein D binds the extracellular domains of Toll-like receptors 2 and 4 through the carbohydrate recognition domain by a mechanism different from its binding to phosphatidylinositol and lipopolysaccharide.. Biochemistry 45:8657-8664, 2006 Abstract
| | Crouch EC, Smith K, McDonald B, Briner D, Linders B, Holmskov U, Hartshorn K.. Species differences in the carbohydrate binding preferences of Surfactant Protein D: Contributions of aspartate-325 and multimeric assembly to ligand recognition.. Am J Respir Cell Mol Biol 35(1):84-94, 2006 Abstract
| | Crouch EC, Whitsett JA.. Diverse Roles of Lung Collectins in Pulmonary Innate Immunity. AB Ezekowitz and JA Hoffmann Editors, Humana Press Inc., Rotowa, NJ. Innate Immunity. 205-229, 2002
| | Crouch E, Wright JR.. Surfactant Proteins A and D and Pulmonary Host Defense. Annu Rev Physiol 63:521-554, 2001 Abstract
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