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Research > Faculty By Division > Jacques Baenziger, MD, PhD

Professor, Pathology and Immunology
Professor, Cell Biology and Physiology
Room 7730, CSRB
Office: (314) 362-8730
E-mail: baenziger@wustl.edu
Pub Med Search

Research

Unique carbohydrate structures have been described on an increasing number of secreted and membrane glycoproteins. Recognition of these structures by highly specific receptors has important biologic consequences. These carbohydrate specific receptors play roles in glycoprotein folding and assembly, inflammation, development, malignant transformation, and targeting. We have identified a new carbohydrate binding motif that mediates recognition of a unique family of carbohydrate structures terminating with the sequence SO4-4-GalNAcâ 1,4GlcNAcâ 1,4Maná (S4GGnM). This receptor has a second, independent binding site for carbohydrates that terminate with mannose (Man). The Man/GalNAc-4-SO4-Receptor is present in a number of different cell types. Its expression is both developmentally and hormonally regulated in a cell specific manner. We are continuing to identify new secreted and membrane glycoproteins that bear oligosaccharides with terminal GalNAc-4-SO4 and are recognized by the Man/GalNAc-4-SO4-Receptor. The Man/GalNAc-4-SO4-Receptor is a multifunctional receptor that is an important model for understanding how unique oligosaccharide structures are utilized for complex biologic processes in vivo. It plays an important role in reproduction in vivo because it recognizes the sulfated carbohydrates on LH, thereby controlling the circulatory half-life of LH. It has different roles in other settings.

The expression of function by receptors like the Man/GalNAc-4-SO4-Receptor requires that unique carbohydrate structures be synthesized on specific glycoproteins. The sulfated carbohydrates on LH are assembled by glycosyltransferases that are protein-specific. Expression of these transferases, like expression of the above receptor, is developmentally and hormonally regulated in a cell specific manner. We are determining at a molecular level how glycosyltransferase expression is regulated during development and during different phases of the hormonal cycle; how expression of the GalNAc-4-SO4-receptor is regulated; and how these structures play a role in recognition events which occur during inflammation and development. Since these carbohydrates and the Man/GalNAc-4-SO4-Receptor play important roles in multiple settings, we are preparing mutant mice deficient in receptor expression to establish the role of this system in vivo. Using these model systems we will define the multiple biologic roles played by this complex system.

Editorial Responsibilities

PresentEditorial BoardJournal of Clinical Investigation
PresentEditorial BoardJournal of Biological Chemistry
PresentEditorial BoardAnalytical Biochemistry

Service to the Department

PresentPathology Course: Lecturer
PresentAutopsy Service: Attending

Service to the University

PresentSteering Committee Molecular Cell Biology
PresentAdmissions Committee DBBS
PresentMolecular Cell Biology Course: discussion section

DBBS Graduate Program Affiliation

Molecular Cell Biology Program
Developmental Biology Program
Biochemistry Program

Diagnostic Expertise

Autopsy service

Clinical Interest

Anatomic and Molecular Pathology - Autopsy Pathology

Selected Publications

Woodworth A, Pesheva P, Fiete D, Baenziger JU.. Neuronal specific synthesis and glycosylation of tenascin-R.. J Biol Chem 279:10413-10421, 2004 Abstract

Park EI, Manzella SM, Baenziger JU. Rapid clearance of sialylated glycoproteins by the asialoglycoprotein receptor. J Biol Chem 278:4597-4602, 2003 Abstract

Baenziger JU. A major step on the road to understanding a unique post-translational modification and its role in a genetic disease.. Cell 113:421-422, 2003 Abstract

Baenziger JU.. Glycoprotein hormone GalNAc-4-sulfotransferase.. Biochem Soc Trans 31:326-330, 2003 Abstract

Roseman DS, Baenziger JU.. Recognition of carbohydrates in biological systems. The Man/GalNAc-4-SO4-receptor: relating specificity to function.. Methods Enzymol 363:121-133, 2003 Abstract

Woodworth A, Fiete D, Baenziger JU.. Spatial and temporal regulation of tenascin-R glycosylation in the cerebellum. J Biol Chem 277:50941-50947, 2002 Abstract

Kang HG, Evers MR, Xia G, Baenziger JU, Schachner M.. Molecular cloning and characterization of chondroitin-4-O-sulfotransferase-3: A novel member of the HNK-1 family of sulfotransferases.. J Biol Chem 277:34766-34772, 2002 Abstract

Mi Y, Shapiro SD, Baenziger JU.. Regulation of lutropin circulatory half-life by the mannose/N-acetylgalactosamine-4-SO4 receptor is critical for implantation in vivo.. J Clin Invest 109:269-276, 2002 Abstract

Evers MR, Xia G, Kang HG, Schachner M, Baenziger JU.. Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase.. J Biol Chem 276:36344-36353, 2001 Abstract

Roseman DS, Baenziger JU.. The mannose/N-acetylgalactosamine-4-SO4-receptor displays greater specificity for multivalent than monovalent ligands.. J Biol Chem 276:17052-17057, 2001 Abstract

Kang HG, Evers MR, Xia G, Baenziger JU, Schachner M.. Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal â 1,4-linked N-acetylgalactosamine.. . J Biol Chem 276:10861-10869, 2001 Abstract

Xia G, Evers MR, Kang H-G, Schachner M, Baenziger JU.. Molecular cloning and expression of the pituitary glycoprotein hormone N-acetylgalactosamine-4-O-sulfotransferase.. J Biol Chem 275:38402-38409, 2000 Abstract

Keusch J, Manzella SM, Nyame KA, Cummings RD, Baenziger JU.. Cloning of Gb3 synthase, the key enzyme in globo-series glycospingolipid synthesis, predicts a family of á 1,4 glycosyltransferases conserved in plants, insects and mammals.. J Biol Chem 275:25315-25321, 2000 Abstract

Keusch J, Manzella SM, Nyame KA, Cummings RD, Baenziger JU.. Expression cloning of a new member of the ABO blood group glycosyltransferases iGb3 synthase, that directs the synthesis of isoglobo-glycosphingolipds.. J Biol Chem 275:25308-25314, 2000 Abstract

Roseman DS, Baenziger JU.. Molecular basis of LH recognition by the Man/GalNAc-4-SO4-receptor.. Proc Natl Acad Sci USA 97:9949-9954, 2000 Abstract

Fiete D, Beranek MC, Baenziger JU.. The cysteine-rich domain of the "mannose" receptor mediates GalNAc-4-SO4 binding.. Proc Natl Acad Sci USA 95:2089-2093, 1998 Abstract

Fiete D, Beranek MC, Baenziger JU.. The macrophage/endothelial cell mannose receptor cDNA encodes a protein which binds oligosaccharides terminating with SO4-4-GalNAcâ1,4GlcNAcâ or Man at independent sites.. Proc Natl Acad Sci USA 94:11256-11261, 1997 Abstract

Manzella SM, Dharmesh SM, Cohick CB, Soares MJ, Baenziger JU.. Developmental regulation of pregnancy-specific oligosaccharide structure, NeuAcá2,6GalNAcâ1,4GlcNAc, on select members of the rat prolactin family.. J Biol Chem 272:4775-4782, 1997 Abstract

Hooper LV, Manzella SM, Baenziger JU.. The biology of sulfated oligosaccharides. Glycosciences: Status and Perspectives (H.-J. Gabi, 1997


Current Trainees

Lindsey Taylor
Nicquet Blake, PhD
Yiling Mi, PhD

Past Trainees

Research Associates
Rajeev Boregowda, PhD